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Anti-CNPase Antibody (A85408) | Antibodies.com

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Added by Admin in Drone jelly (homogenates)


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* Name: Anti-CNPase Antibody
* Description: Rabbit polyclonal antibody to CNPase.
* Applications: WB, ICC/IF, IHC
* Recommended Dilutions: WB: 1:1000-5,000. IF/IHC: 1:1,000-2,000.
* Reactivity: Human, Rat, Mouse
* Immunogen: Full length human recombinant protein.
* Host: Rabbit
* Clonality: Polyclonal
* Isotype: IgG
* Conjugate: Unconjugated
* Purity: Whole antiserum.
* Product Form: Liquid
* Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Figure 1:

Western blot analysis of different tissue lysates using Anti-CNPase Antibody (1:5,000 | red): [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord. Double bands at 46, 48kDa mark correspond to isotypes of the CNP protein. The blot was simultaneously probed with Anti-Calretinin Antibody (A85366 | 1:2,000 | green). The single band at 29kDa corresponds to the calretinin protein.

Figure 2:

Mixed neuron-glial cell cultures stained with Anti-CNPase Antibody (green) and Anti-GFAP Antibody (A85422 | red). The Anti-CNPase Antibody stains strongly in oligodendrocytes, whereas Anti-GFAP Antibody labels the intermediate filament in astrocytes. Blue is DNA staining showing the nuclei of these and numerous other cells, most of which are neurons and which do not express either CNP or GFAP.

Figure 3:

Blot of rat brain tissue homogenates probed with Anti-CNPase Antibody (1:5,000) using LiCor infrared imager. Anti-CNPase Antibody binds strongly and specifically to a band at ~48 kDa. Numbers at left show positions of protein standards of indicated molecular weight in kDa.

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